55,600 research outputs found
Ubiquitin C-terminal hydrolase37 regulates Tcf7 DNA binding for the activation of Wnt signalling
The Tcf/Lef family of transcription factors mediates the Wnt/��-catenin pathway that is involved in a wide range of biological processes, including vertebrate embryogenesis and diverse pathogenesis. Post-translational modifications, including phosphorylation, sumoylation and acetylation, are known to be important for the regulation of Tcf/Lef proteins. However, the importance of ubiquitination and ubiquitin-mediated regulatory mechanisms for Tcf/Lef activity are still unclear. Here, we newly show that ubiquitin C-terminal hydrolase 37 (Uch37), a deubiquitinase, interacts with Tcf7 (formerly named Tcf1) to activate Wnt signalling. Biochemical analyses demonstrated that deubiquitinating activity of Uch37 is not involved in Tcf7 protein stability but is required for the association of Tcf7 to target gene promoter in both Xenopus embryo and human liver cancer cells. In vivo analyses further revealed that Uch37 functions as a positive regulator of the Wnt/��-catenin pathway downstream of ��-catenin stabilization that is required for the expression of ventrolateral mesoderm genes during Xenopus gastrulation. Our study provides a new mechanism for chromatin occupancy of Tcf7 and uncovers the physiological significance of Uch37 during early vertebrate development by regulating the Wnt/��-catenin pathway. ? 2017 The Author(s).113Ysciescopu
Start-to-end modelling of a mode-locked optical klystron free electron laser amplifier
A free electron laser (FEL) in a mode-locked optical klystron (MLOK) configuration is modelled using start-to-end simulations that simulate realistic electron beam acceleration and transport before input into a full three-dimensional FEL simulation code. These simulations demonstrate that the MLOK scheme is compatible with the present generation of radiofrequency accelerator designs. A train of few-optical cycle pulses is predicted with peak powers similar to those of the equivalent conventional FEL amplifier. The role of electron beam energy modulation in these results is explained and the limitations of some simulation codes discussed. It is shown how seeding the FEL interaction using a High Harmonic seed laser can improve the coherence properties of the output
A key to room-temperature ferromagnetism in Fe-doped ZnO: Cu
Successful synthesis of room-temperature ferromagnetic semiconductors,
ZnFeO, is reported. The essential ingredient in achieving
room-temperature ferromagnetism in bulk ZnFeO was found to be
additional Cu doping. A transition temperature as high as 550 K was obtained in
ZnFeCuO; the saturation magnetization at room
temperature reached a value of per Fe. Large
magnetoresistance was also observed below K.Comment: 11 pages, 4 figures; to appear in Appl. Phys. Let
SPEAR Far Ultraviolet Spectral Images of the Cygnus Loop
We present far-ultraviolet (FUV) spectral images, measured at C IV 1550, He
II 1640, Si IV+O IV] 1400, and O III] 1664, of the entire Cygnus Loop, observed
with the Spectroscopy of Plasma Evolution from Astrophysical Radiation (SPEAR)
instrument, also known as FIMS. The spatial distribution of FUV emission
generally corresponds with a limb-brightened shell, and is similar to optical,
radio and X-ray images. The features found in the present work include a
``carrot'', diffuse interior, and breakout features, which have not been seen
in previous FUV studies. Shock velocities of 140-160 km/s is found from a line
ratio of O IV] to O III], which is insensitive not only to resonance scattering
but also to elemental abundance. The estimated velocity indicates that the fast
shocks are widespread across the remnant. By comparing various line ratios with
steady-state shock models, it is also shown that the resonance scattering is
widespread.Comment: 13 pages, 3 figures, 1 table, accepted for publication in ApJ
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